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Cloning and Expression
Enzymes
Hot StartTaq2X Master Mix
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Hot StartTaq2X Master Mix
Hot StartTaq2X Master Mix from New England Biolabs
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Citations:
(10)
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New England Biolabs for
Hot StartTaq2X Master Mix
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Citations
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Product Specs
Item
Hot StartTaq2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0496L
Quantity
500 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (10)
(1)
Genome Sequence of Fowlpox Virus-Integrated Reticuloendotheliosis Virus from a Rio Grande Wild Turkey (Meleagris gallopavo
intermedia
)
Microbiology Resource Announcements
June 16, 2022
Bianca Willis, Camille Trautman, Faith Cox, Tiffany Lujan, Jason Hardin, Robert Dittmar, Camila Romano, Jeff Brady, Dustin Edwards
isolate TSU-1029 contained an REV proviral genome insertion,
PCR
using Hot Start Taq 2× master mix (
NEB
;
M0496L
) and the heterologous primers REV env 7F and FPV 203 4R produced an appropriately sized 740-bp product
(2)
NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes
Nucleic acids research
May 20, 2022
Bao H, Carraro M, Flury V, Liu Y, Luo M, Chen L, Groth A, Huang H.
10 minutes. 2 μl of this lysate was directly used for
PCR reactions
with 2 × Taq start master mix (
M0496L
,
NEB
), and the two
NASP
clone
screening
primers (see primer table). Amplified DNA was sequenced using
(3)
Reconstructing the Lineage Histories and Differentiation Trajectories of Individual Cancer Cells in Myeloproliferative Neoplasms
Cell stem cell
March 4, 2021
Van Egeren D, Escabi J, Nguyen M, Liu S, Reilly CR, Patel S, Kamaz B, Kalyva M, DeAngelo DJ, Galinsky I,
et al
.
previously. In Step 3, a 25uL
PCR
mixture was made containing 12.5uL of Hot Start
Taq 2x Master Mix
(
NEB
#
M0496S
), 1.25uL of cDNA additive (10x Genomics #220067), 1.25uL of forward primer (Partial P5, AATGATACGGCGACCACCGAGATCT)
(4)
Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP)
FASEB BioAdvances
March 1, 2020
Julian Rubinfien et al.
Q5 Hot Start High‐Fidelity 2X Master Mix
(
NEB
M0494
; New England Biolabs, Inc) or Hot Start
Taq 2X Master Mix
(
NEB
M0496
; New England Biolabs, Inc), along with 5 μL primers prepared at 5 μmol/L concentration, 18 μL distilled
(5)
Recovery of Renal Function following Kidney-Specific VEGF Therapy in Experimental Renovascular Disease
American journal of nephrology
January 1, 2020
Engel JE, Williams ML, Williams E, Azar C, Taylor EB, Bidwell GL, Chade AR.
(
Thermo Scientific
,
K1621
).
qPCR
was performed using Hot Start
Taq 2x Master Mix
(
New England BioLabs
,
M0496L
). Samples were probed using primer pairs for Beta Actin (Hs01060665_g1), VEGF (Hs00900055_m1), KDR/Flk-1
(6)
Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS
Cell
December 12, 2019
Chao CC, Gutiérrez-Vázquez C, Rothhammer V, Mayo L, Wheeler MA, Tjon EC, Zandee SEJ, Blain M, de Lima KA, Takenaka MC,
et al
.
DAPI Fluoromount-G
,
SouthernBiotech
,Cat#
0100–20
NuPAGE LDS sample buffer,
Invitrogen
,Cat#
NP0007
Hot Start
Taq 2X Master Mix
,
New England Biolabs
,Cat#
M0496
FuGENE HD Transfection Reagent
,
Promega
,Cat#
E2311
UK-5099
,
Tocris Bioscience
,Cat#4186 methylglyoxal
(7)
Demonstration of End-to-End Automation of DNA Data Storage
Scientific Reports
December 1, 2019
Christopher N. Takahashi, Bichlien H. Nguyen, Karin Strauss, Luis Ceze
kilobase fragment that was
PCR
-amplified from the lambda genome using hot start TAQ DNA polymerase (
NEB
M0496
) with a Bsa-I restriction site added by the forward primer. The resulting fragment after digestion had
(8)
Aptamers in the Therapeutics and Diagnostics Pipelines
Theranostics
July 1, 2018
Harleen Kaur, John G. Bruno, Amit Kumar, Tarun Kumar Sharma
enzymes and other molecular biology reagents, has commercialized Hot Start Taq DNA polymerase (Cat No.
M0496S
and
M0490S
). Both of these products principally rely on an aptamer-based hot start technology to avoid
(9)
Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer
Journal of Bacteriology
April 1, 2017
Anthony W. Kingston, Christine Ponkratz, Elisabeth A. Raleigh
(catalog number
M0491
;
NEB
), while diagnostic PCRs used the Hot Start Taq 2× master mix (catalog number
M0496
;
NEB
). Matings. Donor and recipient cultures were grown at 37°C to an optical density at 600 nm (OD600)
(10)
Novel recA-Independent Horizontal Gene Transfer in Escherichia coli K-12
PLOS ONE
July 10, 2015
Anthony W. Kingston, Chloé Roussel-Rossin, Claire Dupont, Elisabeth A. Raleigh
(#
M0491
;
New England Biolabs
) while diagnostic
PCR reactions
used the Hot Start
Taq 2X Master Mix
(#
M0496
;
New England Biolabs
). New England Biolabs produced all restriction enzymes and ligases used in these
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Hot StartTaq2X Master Mix from New England Biolabs
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